HEK-Blue™ hTLR4

1.212,00 €

Human TLR4-expressing HEK 293 cells 3-7 x 10e6 cells

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Human TLR4 / NF-κB / SEAP reporter HEK293 cells

HEK-Blue™-hTLR4 cells were obtained by co-transfection of the human TLR4, MD-2 and CD14 co-receptor genes, and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.

The SEAP reporter gene is placed under the control of an IL-12 p40 minimal promoter fused to five NF-κB and AP-1-binding sites.

Stimulation with a TLR4 ligand activates NF-κB and AP-1 which induce the production of SEAP.

Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.

The parental cell line of HEK-Blue™ hTLR4 cells is HEK-Blue™ Null2 cells.

hkb-htlr4

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hkb-htlr4

hkb-htlr4

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