IBIAN-TAQ DNA POLYMERASE, 5 U/ - (500 U)

Description

IBIAN®-Taq DNA Polymerase is a recombinant Taq DNA Polymerase, suitable for various PCR applications. The enzyme possesses a 5’ - 3’ polymerase activity and generates 3’A-overhangs. The PCR products obtained with IBIAN-Taq DNA Polymerase are free of unspecific products and primer-dimers. For HotStart applications an antibody blocked variant is available.

Shipping and Storage Conditions

Product is shipped on blue-ice.

Store at -20 °C Avoid repeated freeze/thaw cycles. can be IBIAN-Taq Polymerase can be stored at 2°C to 8°C for short-term storage.

 Content and Order information

We strongly suggest you starting with the black cup buffer: Complete KCl Reaction Buffer (10x)

ª Incomplete NH₄ Reaction Buffer (10x): pH 8.8, 0.1% Tween 20, free of MgCl₂.

^b Complete NH₄ Reaction Buffer (10x): pH 8.8, 0.1% Tween 20, 20 mM MgCl₂.

^c *Complete KCl Reaction Buffer (10x): pH 8.8, 0.1% Tween 20, 15 mM MgCl₂ .

Performance and purity tests

IBIAN®-Taq DNA Polymerase effectively directs PCR with templates up to 5 kb in length. The enzyme was tested for the absence of exo-/endonuclease activities. No traces of bacterial DNA were detected in PCR reaction with “no template” test with the primers complementary to the conservative region of 16S ribosomal gene.

The following tests are performed with each lot of IBIAN-Taq DNA Polymerase:

·       PCR amplification tests with different templates

·       No detectable exo-/endonuclease activities

·       “no primers” test with Lambda DNA cycling without primers

 Applications

IBIAN®-Taq DNA Polymerase is suitable for all regular PCR applications, especially for complex genomic or cDNA templates, low copy number targets, Multiplex and Real-Time PCR. This polymerase effectively amplifies templates up to 5 kb length.

 Sensitivity

High sensitivity of PCR reactions with IBIAN®-Taq DNA polymerase in the optimal conditions – in some reactions at least 10 DNA molecules are necessary for detection. Enzyme has a very good performance in single-copy gene PCR from genomic mammalian DNA. In contrast to the IBIAN enzyme, Taq DNA polymerases from the variety of suppliers contain contaminating DNA. With DNA-contaminated Taq DNA polymerase you can observe false-positive PCR results in some cases.

Unit definition: One unit of activity is defined as the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble DNA fraction in 30 minutes at 72 °C.

 Recommended MgCl₂ concentration 1.5 mM – 6 mM. Available our excel table “How to prepare your Master-Mix”

 Storage buffer:

Enzyme is supplied in 10mM K3PO4 pH 7.4, 0.1mM EDTA, 50% glycerol, 0.1% Triton X-100, 0.1% Tween-20.