IBIAN-TAQ DNA POLYMERASE, 5 U/ - (500 U)

IBIAN®-Taq DNA Polymerase is a thermostable enzyme of approximately 94 kDa, isolated from eubacterium Thermus aquaticus strain YT-1 (1). This unmodified enzyme replicates DNA at 72°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5' -> 3' direction in the presence of magnesium ions and shows 5' -> 3' exonuclease activity. The enzyme is highly purified and free of nonspecific endo- or exonucleases. IBIAN®-Taq DNA Polymerase leaves single 3' dA nucleotide overhangs on their reaction products.

Concentration: 5 units/µl

Unit definition:

One unit of activity is defined as the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble DNA fraction in 30 minutes at 72 °C.

Applications:

IBIAN®-Taq DNA Polymerase is suitable for all regular applications - PCR, primer extension reactions etc. IBIAN®-Taq is free of bacterial DNA and therefore suitable for the work with bacterial DNA.

Sensitivity:

High sensitivity of PCR reactions with IBIAN®-Taq DNA polymerase in the optimal conditions - in some reactions at least 6 DNA molecules are necessary for detection. Enzyme has a very good performance in single-copy gene PCR from genomic mammalian DNA. In contrast to the IBIAN® enzyme, Taq DNA polymerases from the variety of suppliers contain contaminating DNA. With DNA-contaminated Taq DNA polymerase you can observe false-positive PCR results in some cases.