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Description
IBIAN®-HighHotTaq DNA Polymerase is a thermostable enzyme of approximately 94 kDa, isolated
from eubacterium Thermus aquaticus strain YT-1 bound to anti-Taq DNA polymerase monoclonal antibodies. Polymerase activity is blocked during set-up of the PCR at ambient temperature (20 – 22 °C). The inhibition is completely reversed when the temperature is increased above 70 °C The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5' → 3' direction in the presence of magnesium ions and shows 5' → 3' exonuclease activity. The enzyme is highly purified and free of nonspecific endo- or exonucleases.
Shipping and Storage Conditions
Product is shipped on blue-ice. Store at -20 °C Avoid repeated freeze/thaw cycles. Reagent for in vitro laboratory use only
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